The effect of synthesized chitosan grafted poly (N-L-lactide) on human genetic material


Introduction: Chitosan is one of the natural polymers can generally consider as a biocompatible and biodegradable polycationic polymer, which has minimum immunogenicity and low cytotoxicity. Therefore, chitosan and its derivatives may represent potentially safe cationic carriers for use in gene delivery.Materials and Methods: Chitosan with 90.1 DD% obtained by deacetylation of chitin extracted from local shrimp shells. Graft copolymerization of L-lactide onto chitosan was carried out at room temperature by ring opening polymerization under a nitrogen atmosphere to prepare chitosan-g-poly (N-lactide) graft copolymer. It was obtained in good yield and characterized by FTIR. The samples purity and concentration were detected using both Nanodrop UV-spectroscopy and agarose gel electrophoresis techniques. The human heat shock proteins gene, hsp-70, was used as a model of human genes to study the effect of chitosan-g-poly(N-lactide) graft copolymer. Results and Discussion: The results revealed that chitosan-g-poly (N-lactide) graft copolymers had safety effect on the DNA, and binding with it. the human heat shock proteins gene, hsp-70, was used as a model of human genes to study the effect of chitosan-g-poly(N-lactide) graft copolymer, it shows a good binding ability the human gene, implies that it might be used in biomedical applications in the future. Conclusions: Grafting of L-lactide onto chitosn by ring opening polymerization was confirmed by FTIR.   The repaired polymer has safety effects on human DNA and genes. The chitosan-g-poly (N-lactide) graft copolymer has shown high efficiency to electrostatic interaction with human DNA and gene, implying that it is suitable to be used as DNA and gene delivery.


Bavariya AJ, Andrew Norowski P Jr, Mark Anderson K, Adatrow PC, Garcia-Godoy F, Stein SH, Bumgardner JD.Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin. J. Biomed. Mater. Res. B Appl. Biomater. 2014;102(5): 1084-92.

Wang J, Wang L, Zhou Z, Lai H, Xu P, Liao L, Wei J. Biodegradable polymer membranes applied in guided bone/tissue regeneration: A review. Polymers.2016;8: 115-34.

Zimoch-Korzycka A, Kulig D, Jarmoluk A, Marycz K, Matuszczak W. Study of enzymatically treated alginate/chitosan hydrosols in sponges formation process. Polymers. 2016; 8(1): 8-20.

Avérous L, Pollet E, Editors.Biodegradable polymers. Springer-Verlag, London;2012.

Elnashar M. Editor. Biotechnology of biopolymers. Intech Open Publisher. 2011.

Puvvada Y S, Vankayalapati S, Sukhavasi S. Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. Intern Curr Pharma J.2012;1(9): 258-63.

Mansouri S, Cuie Y, Winnik F, Shi Q, Lavigne P, Benderdour M, Beaumont E, Fernandes J.C. Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials,2006; 27(9): 2060-5.

Jiang H L , Kim Y K , Cho, C S , Cho M H. Medical Polymer-based gene therapy, Non-viral gene therapy, Xbo Yuan editord. Intech Publisher. 2011.

Lavertu M , Methot S , Tran-Khanh N , Buschmann M D . High effficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials. 2006; 27: 4815-24.

Luckachan G E , Pillai C K S. Chitosan/Oligo L-lactide Graft Copolymers: Effect of Hydrophobic Side Chains on the Physico-Chemical Properties and Biodegradability. Carbohydrate Polymers 2006; 64: 254-266.

Mahajan A, Aggarwal G. Smart polymers: Innovations in novel drug delivery. Int. J. Drug Dev. Res. 2011; 3(3): 16-30.

Liua P , Hua Y , Fana Z , Li S. Synthesis, characterization and self-assembly behaviour of chitosan-graft polylactide copolymers. Nanoscience & Nanotechnology-Asia. 2012; 2: 38-46.

Manavitehrani I, Fathi A, Badr H, Daly S, Shirazi A N , Dhegihan N. Biomedical applications of biodegradable polyesters. Polymers. 2016;8: 20-51.

Wu Y , Zheng Y , Yang W , Wang C , Hu J , Fu S. Synthesis and characterization of a novel amphiphilic chitosan-polylactide graft copolymer. Carbohydrate Polymers. 2005; 59:165-171.

Kim J Y , Ha C S , Jo N J. Synthesis and properties of biodegradable chitin-graft-poly(L-lactide) copolymers, Polym. Int. 2002;51: 1123-8.

Liu L , Shi A , Guo S , Fang Y , Chen S , Li J. Preparation of chitosan-g-polylactide graft copolymers via self-catalysis of phthaloylchitosan and their complexation with DNA, Reactive & Functional Polymers. 2010;70(5) :301-5.

Yao F L , Liu C , Chen W , Bai Y , Tang Z Y , Yao K D . Synthesis and characterization of chitosan grafted oligo(L-lactic acid). Macromolecular Bioscience. 2003;3(11): 653-6.

Feng H , Dong, C M Preparation, characterization, and self-assembled properties of biodegradable chitosan-poly(L-Lactide) hybrid amphiphiles. Biomacromolecules. 2006;7: 3069-75.

Kaya M , Baran T , Erdogan S , Mentes A , Asan Özüsaglam M, Çakmak, Y S Physicochemical comparison of chitin and chitosan obtained from larvae and adult Colorado potato beetle (Leptinotarsa decemlineata). Mater. Sci. Eng. C , Mater. Biol. Appl. 2014, 45: 72-81.

Mutasher S H , Saleh A A , Al-Lami H S Preparation of some chitosan derivatives and study their effect on human genetic material, Der Pharma Chemica. 2016; 8(11):125-34.

PuvvadaY , Vankayalapati S , Sukhavasi S. Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry, Intern Curr Pharm J, 2012;1(9): 258-63.

Bradley D , Williams G , Lawton M. Drying of organic solvents: Quantitative evaluation of the efficiency of several desiccants. J. Org. Chem. 2010;75(24): 8351-4.

Kowalczyk A , Guzik K , SlezakK , DziedzicJ , Rokita H. Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophages. J. Inflammation. 2005;2 : 12-21.

Silverstien R M , Webster F X , Kiemle D J. Editors. Spectrometric identification of organic compounds, 7th edition. John Wiley & Sons Inc., New York; 2005.

Harpe A , Petersen H , Li Y , Kissel T. Characterization of commercially available and synthesized polyethylenimines for gene delivery. J. Control. Release. 2000;69: 309-22.

Bronich T K , Kabanov A V , Marky L A.A thermodynamic characterization of the interaction of a cationic copolymer with DNA. J. Phys. Chem. B. 2001;105: 6042-50.

Senthil Kumar R , Sasikala K , Arunachalam S. DNA Interaction of some polymer-copper(II) complexes containing 2,20-bipyridyl ligand and their antimicrobial activities, J. Inorg. Biochem.2008; 102: 234-41.

Mao S R, Sun W , Kissel T. Chitosan-based formulations for delivery of DNA and siRNA. Adv Drug Deliv Rev. 2010; 62 :12-27.

Ahn C H , Chae S Y , Bae Y H , Kim S W. Biodegradable poly(ethylenimine) for plasmid DNA delivery. J. Control. Release. 2002;80: 273-82.

Forrest M L , Koerber J T , Pack D W. A degradable polyethylenimine derivative with low toxcicity for highly efficient gene delivery. Bioconjugate Chem. 2003; 14: 934-40.

De Smedt S C , Demeester J, Hennink W E Review: cationic polymer based gene delivery systems. Pharmaceutical Research.2000; 17(2): 113-26.

Rungsardthong U , Ehtezazi T , Bailey L , Armes S P , Garnett M C , Stolnik S. Effect of polymer ionization on the interaction with DNA in nonviral gene delivery systems. Biomacromolecules. 2003;4: 683-90.

Cómo citar
Al-Lami, H. S., saleh, A. A., Jalal, M. A., & Mutasher, S. H. (2018). The effect of synthesized chitosan grafted poly (N-L-lactide) on human genetic material. Revista Innovaciencia , 6(1), 1-10.
Artículo de investigación científica y tecnológica