Detección de Alfa 1-antitripsina e Iinterleucina-8 en pacientes con Meningitis

Autores/as

  • Samir K. Abbas B.Sc. in Biology, High Diploma student in Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University
  • Mayada N. Iqbal Assist. Prof. PhD in Immunology, Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University
  • Nahlah G. Abdul Majeed Consultant PhD, Immunology laboratory, Teaching laboratories, Baghdad.

DOI:

https://doi.org/10.15649/2346075X.656

Palabras clave:

Meningitis; IL-8; Alpha1antitrypsin; CSF

Resumen

Meningitis is a meninges inflammation and the subarachnoid cerebrum spinal fluid that surrounds the spinal cord and brain. Infantile meningitis
is a major cause of morbidity & mortality in various parts of the world; it is earnest disorder owing to the nearness of the location to the brain and
spinal cord. It can be caused by virtually any organism; in general viral infections are more common than bacterial, fungal and parasitic infections
respectively. Objective: investigation of the Interleukin-8 (IL-8) and Alaph-1 Antitrypsin in the cerebrum spinal fluid of meningitis patients.
Material and Method: A study was done at the Medical city and Al-kadimia hospitals included 47 and 20 beings of Patients and Control groups
with an overall age range between 11 days to 6 years old. According to the causative agents of the disease, they were divided into three groups:
bacterial, viral and control groups. All subjects were monitored for the IL-8 by Enzyme Linked Immunosorbent Assay (ELISA) and Alaph-1
Anti-trypsin by Single Radial Immunodiffusion assay (SRIA) in the cerebrospinal fluid the data was analyzed via using SPSS (chi-squire test)
program (version 22). Results and discussion: The results revealed the presence of an elevation concentration of IL-8 among meningitis disease
patients (484.0695 pg/ml ), high concentration of IL-8 seemed in bacterial meningitis (825.3250pg/ml) when compared with its level in viral
meningitis (142.8143pg ml) ( p= 0.002) and control group ( 56.3409 pg/ml) (p=0.00) but no significant difference was found between viral and
control groups (p= 0.679). Alpha 1 antitrypsin results appeared high concentration of (21.6462 mg/ dl) and (16.4000 mg/ dl) in bacterial and viral
meningitis groups in sequentially, and that concentrations showed differences arrived significantly. The statistical analysis of this research showed
a correlation between IL-8 levels and both of neutrophil and lymphocyte in meningitis patients. IL-8 had significant correlation with lymphocyte
(p=0.02) in viral group while no significant correlation was appeared between IL-8 and neutrophil. In bacterial group, lymphocytes with IL-8 had
weak correlation, in contrary the correlation between neutrophil and IL-8 revealed the significantly correlation (p=0.01). No correlation was establish concerning the Alpha-1 antitrypsin and the cell’s numbers and types. Conclusion: A study indicated that Alpha 1 antitrypsin and IL-8 played
an important role in meningitis disease.

Biografía del autor/a

Samir K. Abbas, B.Sc. in Biology, High Diploma student in Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University

B.Sc. in Biology, High Diploma student in Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University.

Mayada N. Iqbal, Assist. Prof. PhD in Immunology, Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University

Assist. Prof. PhD in Immunology, Department of Medical Laboratory Technology, College of Health and Medical Technology, Middle Technical University.

Nahlah G. Abdul Majeed, Consultant PhD, Immunology laboratory, Teaching laboratories, Baghdad.

Consultant PhD, Immunology laboratory, Teaching laboratories, Baghdad.

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2019-10-29

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Abbas, S. K. ., Iqbal, M. N. ., & Abdul Majeed, N. G. . (2019). Detección de Alfa 1-antitripsina e Iinterleucina-8 en pacientes con Meningitis. Innovaciencia, 7(1), 1–10. https://doi.org/10.15649/2346075X.656

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Artículo de investigación científica y tecnológica

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