Contaminación fúngica de frutos secos de vid y detección de ocratoxina en jugo de uva enDuhok, Iraq

Autores/as

  • Asia A. M. Saadullah Assistant Professor, Biology Department, College of Science, University of Duhok, Iraq
  • Samir K. A bdullah Professor, Medical Laboratory Technology Department, Alnoor University College, Nineva, Iraq

DOI:

https://doi.org/10.15649/2346075X.472

Palabras clave:

Ochratoxin A; Dried vines fruit; Grape juice; Contamination; Fungi; Iraq

Resumen

Introduction: Dried vine fruits (raisins) and their juice are widely consumed by human as a diet. Raisins have been shown highly contaminated with ochratoxin A (OTA) and OTA-producing fungi. Ochratoxin A is a potent nephrotoxic and carcinogen to human and animals. Materials and Methods: Dried vine fruit samples was obtained from local shops for fruit juice and soft drinks in Duhok province. Two different media, Dichloran R ose Ben gal Chloromphenicol Agar (DRBC) and Dichloran 18 % Glycerol Agar (DG-18) was used for the counting and isolation of fungi from dried vine fruits. Grape juice were prepared from dried vine fruit after blending with water in a commercial blender. Natural contamination with ochratoxin A was detected by LC-MS/MS technique. Results and Discussion: All samples confirmed to be contaminated with fungi with various degrees. A total of 19 filamentous genera of fungi as well to Yeasts and non sporulation mycelium was detected. Predominant genera detected on both media were Aspergillus and Penicillium. Detected value of ochratoxn A in juices obtained from dried vine fruits was between 0.37 ng/ml to 1.85 ng/ml. Samples contaminated with ochratoxin A were associated with
Aspergillus carbonarius, A. niger aggregate, A. sclerotium, A. ochraceus, and Penicillum verrucosum. Conclusion: Dried vines fruit were highly contaminated with a broad spectrum of filamentous fungi. Black aspergilli were the most detected species from samples naturally contaminated with ochratoxin A

Biografía del autor/a

Asia A. M. Saadullah, Assistant Professor, Biology Department, College of Science, University of Duhok, Iraq

Assistant Professor, Biology Department, College of Science, University of Duhok, Iraq

Samir K. A bdullah, Professor, Medical Laboratory Technology Department, Alnoor University College, Nineva, Iraq

Professor, Medical Laboratory Technology Department, Alnoor University College, Nineva, Iraq

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Publicado

2018-12-28

Cómo citar

M. Saadullah, A. A. ., & bdullah, S. K. A. (2018). Contaminación fúngica de frutos secos de vid y detección de ocratoxina en jugo de uva enDuhok, Iraq. Innovaciencia, 6(2), 1–8. https://doi.org/10.15649/2346075X.472

Número

Vol. 6 Núm. 2 (2018)

Sección

Artículo original de investigación e innovacion
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