Patogenicidad de especies de Fusarium asociados a daño del ápice y la raíz del trigo (Triticum aestivum) bajo diferentes condiciones

Autores/as

  • Bareen Sidqi Shareef Al-Tovi Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq
  • Raed Abduljabbar Haleem Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq.

DOI:

https://doi.org/10.15649/2346075X.659

Palabras clave:

Fusarium species; Wheat; Pathogenicity; Invivo; In-vitro; Crown and root rot

Resumen

This study was conducted to test the pathogenicity of Fusarium species, the causes of crown and root rot disease of wheat crop, under three different conditions (Laboratory, Greenhouse and Field) and to show the best method for pathogenicity among different conditions. Pathogenicity test of six isolates of Fusarium species (F. graminearum, F. oxysporum, F. avenaceum, F. nivale, F. solani and F. udum) was tested on durum (Simeto) cultivar of wheat by test tube method in the laboratory, the tested fungi had substantial effect on seed germination. F. oxysporum showed the highest germination failure (44.44%) which significantly differed with other species. In the greenhouse, seedlings were inoculated by spore suspension at the base of each plant stem. The most virulent fungus after 35 days of inoculation was F. oxysporum (0.78) followed by F. solani (0.70) and F. graminearum (0.66), while the lowest disease severity was recorded by F. udum (0.16). Also in the field pathogenicity experiments of three Fusarium species (F. graminearum, F. oxysporum and F. solani) were performed on a durum (Simeto) and soft (Cham6) cultivars. Spore suspension was applied at the 2- to 3-leaf Zadoks’s growth stage. Disease severity was calculated at two stages of wheat growth (Booting and Ripening).The most virulent fungus was F. graminearum (0.42) that was significantly different from  other fungi. This work indicated that F. graminearum, F. oxysporum and F. solani showed higher infection than remaining tested species under three
conditions. Pathogenicity test in laboratory by test tube method (In-vitro) appeared more effective than greenhouse and field experiments

Biografía del autor/a

Bareen Sidqi Shareef Al-Tovi, Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq

Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq

Raed Abduljabbar Haleem, Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq.

Plant Protection Department, College of Agriculture, University of Duhok, Duhok Province, Kurdistan Region –Iraq.

Referencias

Paulitz T. C., Smiley R. W. and Cook R. J. Insights into the prevalence and management of soilborne cereal pathogens under direct seeding in the Pacific Northwest, USA. Can. J. of Plant Pathol., 2002; 24(4): 416-428. https://doi.org/10.1080/07060660209507029

Burgess L.W., Backhouse D., Summerell B. A. and Swan L. J., Chapter 20 in Fusarium- Paul E. Nelson Memorial Symposium. Edited by Summerell B.A., Leslie J.F., Backhouse D. Bryden W.L and Burgess L.W. APS Press, The American Phytopathological Society, St. Paul, Minnesota, United States of America. 2001; ISBN 0-89054-268-6. pp. 271-294.

Cook, R. J., Fusarium root, crown, and foot rots and associated seedling diseases. Compendium of wheat diseases and pests. 3rd ed. The Pennsylvania State University Press, University Park, Pennsylvania, USA, 2010; 37-39.

Mergoum, M., Lyamani, A.andNsarellah, N., Root rot of wheat. Al Awamia Magazine National Government publication Morocco,1995; 89:1-24.

Nicol, J. M., Bagci, A., Hekimhan, H., Bolat, N., Braun, H. J. and Trethowan, R., Strategy for the identification and breeding of resistance to dry land root rot complex for international spring and winter wheat breeding programs. Page 283 in: Proc. 4th Int. Crop Sci. Congr., Brisbane, Australia, 2004.

Beev, G., Denev, S. and Pavlov, D., Occurrence and distribution of Fusarium species in wheat grain. Agricul. Scie. and Technol. 2011; 3(2):165-168.

Chakraborty, S., Obanor, F., Westecott, R. and Abeywickrama, K., Wheat crown rot pathogens Fusarium graminearumand F. pseudograminearum lack specialization. Phytopathology, (2010); 100:1057-1065. https://doi.org/10.1094/PHYTO-01-10-0007.

Al-Tovi, B.S., Prevalence and Molecular identification of Fusarium Crown and Root Rot of Wheat in Sumel-Duhok Province. M.Sc. Thesis, College of Agriculture, University of Duhok,Kurdistan region-Iraq. 2017.

Hameed, M. A., Rana, R. M. and Ali, Z., Identification and characterization of a novel Iraqi isolate of Fusarium pseudograminearum causing crown rot in wheat. Genet. Mol. Res, 2012; 11(2): 1341-1348. https://doi.org/10.4238/2012.May.15.4.

Khalifah, M.H., Molecular identification of wheat crown rot pathogen and evaluation of some protective methods. M.Sc. thesis, College of Agriculture, University of Baghdad, Baghdad, Iraq. 2013; (In Arabic).

Taha, Z.A., Wheat root rot in Ninevah government and its biological control. M.Sc. thesis, College of Agriculture and Forestry, University of Mosul, Iraq. 2014; (In Arabic).

Gargouri-Kammoun, L., Gargouri, S., Rezgui, S., Trifi, M., Bahri, N.andHajlaoui, M. R., Pathogenicity and aggressiveness of Fusarium and Microdochium on wheat seedlings under controlled conditions. Tunisian J. of Plant Prot., 2009; 4(2):135-144.

Brennan, J.M., Fagan, B., van Maanen, A., Cooke, B.M. and Doohan, F.M., Studies on in-vitro growth and pathogenicity of European Fusarium fungi. Eur. Journal of Plant Pathology.(2003); 109: 577-587. https://doi.org/10.1023/A:1024712415326.

Carter, J. P., Rezanoor, H. N., Holden, D., Desjardins, A. E., Plattner, R. D. and Nicholson, P., Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum. Eur. J. of Plant Pathol., 2002; 108(6): 573-583. https://doi.org/10.1023/A:1019921203161.

Tunali, B., Nicol, J., YeldaErol, F. and Altiparmak, G., Pathogenicity of Turkish crown and head scab isolates on stem bases on winter wheat under greenhouse conditions. Plant Pathol. J. 2006; 5: 143-149. https://doi.org/10.3923/ppj.2006.143.149

Booth, C., Fusarium, Laboratory guide to the identification of the major species. Common wealth Mycological Institute. Academic press. UK, 1977; ISBN 0 85198 3839.

Nelson, P.E., Dignani, M.C. and Anaissie, E.J., Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews, 1994;7(4): 479-504. https://doi.org/10.1128/CMR.7.4.479.

Summerell, B.A., Salleh, B. and Leslie, J.F., A utilitarian approach to Fusarium identification. Plant Disease, 2003; 87(2): 117-128. https://doi.org/10.1094/PDIS.2003.87.2.117.

Leslie, J.F. and Summerell, B.A., Techniques and methods. Fusarium laboratory manual. Blackwell Publishing Ltd., Oxford, UK; 2006. https://doi.org/10.1002/9780470278376

Asad, S.I., Shamim. A. and Iftikhar, A., Characterization of Bipolaris sorokiniana isolated from different agro-ecological zones of wheat production in Pakistan. Pakistan. Journal Botany, 2009; 41(1): 301-308.

McKinney, H. H., Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. J. of Agricult. Res., 1923; 26(5), 195-218.

Mitter, V., Zhang, M. C., Liu, C. J., Ghosh, R., Ghosh, M. and Chakraborty, S., A high‐throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathology, 2006; 55(3): 433-441. https://doi.org/10.1111/j.1365-3059.2006.01384.x.

Zadoks, J. C., Chang, T. T. and Konzak, C. F., A decimal code for the growth stages of cereals. Weed Res., 1974;14(6): 415-421. https://doi.org/10.1111/j.1365-3180.1974.tb01084.x.

SAS., SAS/STAT User's Guide, Version 8.2, 1stprinting.Vol. 2. SAS Institute Inc, SAS Campus Drive, Cary, North Carolina, USA, 2003.

Bechtel, D. B., Kaleikau, L. A., Gaines, R. L. and Seitz, L. M.,The effects of Fusarium graminearum infection on wheat kernels. Cereal Chemistry, 1985; 62(3):191-197.

Akinsanmi, O. A., Mitter, V., Simpfendorfer, S., Backhouse, D. and Chakraborty, S., Identity and pathogenicity of Fusarium spp. isolated from wheat fields in Queensland and northern New South Wales. Aust. J. Agric. Res. 2004; 55: 97-107. https://doi.org/10.1071/AR03090

Humphreys, J., Cooke, B. M. and Storey, T., Effect of seed borne Microdochium nivaleon establishment and grain yield. Plant Varieties and Seeds 1995; 8:107-117.

Debnath, M., Sultana, A. and Rashid, A. Q. M. B., Effect of Seed-borne Fungi on the Germinating Seeds and their Bio-control in Maize. Journal of Environ. Sci. and Natural Res., 2012; 5(1):117-120. https://doi.org/10.3329/jesnr.v5i1.11564.

Rodriguez-Galvez, E. and Mendgen, K., .The infection process of Fusarium oxysporum in cotton root tips. Protoplasma, 1995; 189(1-2): 61-72. https://doi.org/10.1007/BF01280291.

Deacon J. W. and Donaldson S.P., Molecular recognition in the homing responses of zoosporic fungi, with special reference to Phytium and Phytophthora. Mycol Res 1993; 97:1153-1171. https://doi.org/10.1016/S0953-7562(09)81278-1.

Brennan, J.M., Fagan, B., van Maanen, A., Cooke, B.M. and Doohan, F.M., Studies on in-vitro growth and pathogenicity of European Fusarium fungi. Eur. J. of Plant Pathol.. 2003; 109: 577-587. https://doi.org/10.1023/A:1024712415326.

Matny, O. N., Chakraborty, S., Obanar, F. and AL-Ani, R. A., Molecular identification of Fusarium spp causing crown rot and head blight on winter wheat in Iraq. J. of Agricult. Technol., 2012; 8(5): 1677-1690.

Wang, Q., The pathosystem wheat (Triticum aestivum) root-Fusarium graminearum: complex plant responses and fungal strategies, Ph.D. dissertation, Justus Liebig University Giessen, Germany. 2015.

Kane, R. T., Smiley, R. W. and Sorrells, M. E., .Relative pathogenicity of selected Fusarium species and Microdochium bolleyito winter wheat in New York. Plant Dis. 1987; 71:177-181. https://doi.org/10.1094/PD-71-0177.

Fernandez, M. R. and Chen, Y., Pathogenicity of Fusarium species on different plant parts of spring wheat under controlled conditions. Plant Dis., 2005; 89(2): 164-169. https://doi.org/10.1094/PD-89-0164.

Jenkinson, P. and Parry, D. W., Isolates of Fusarium species from common broadleaved weeds and their pathogenicity to winter wheat. Mycol. Res. 1994; 98:776-780. https://doi.org/10.1016/S0953-7562(09)81054-X.

Arseniuk, E., Goral, T. and Czembor, H. J., Reaction of triticale, wheat and rye accessions to graminaceous Fusariums pp. infection at the seedling and adult plant growth stages. Euphytica 1993; 70:175-183. https://doi.org/10.1007/BF00023757

Specht, L. P. and Rush, C. M., Fungi associated with root and foot rot of winter wheat and populations of Cochliobolus sativus in the Texas Panhandle. Plant Dis. 1988; 72:959-963. https://doi.org/10.1094/PD-72-0959.

Hajieghrari, B., Wheat crown and root rotting fungi in Moghan area, Northwest of Iran. Afri. J. of Biotechnol., 2009; 8(22):6214-6219. https://doi.org/10.5897/AJB09.1087.

Gordon, T. R., Okamoto, D. and Jacobson, D. J. colonization of muskmelon and no susceptible crops by Fusarium oxysporumf. sp. melonis and other species of Fusarium. Phytopathology, 1989; 79(10):1095-1100. https://doi.org/10.1094/Phyto-79-1095.

Chekali, S., Gargouri, S., Berraies, S., Gharbi, M.S., Nicol, M.J. and Nasraoui, B., Impact of Fusarium foot and root rot on yield of cereals in Tunisia. Tunisian J. of Plant Protec.t 2013; 8: 75-86.

Papendick, R. I. and Cook, R. J., Plant water stress and development of Fusarium foot rot in wheat subjected to different cultural practices. Phytopathology 1974; 64:358-363. https://doi.org/10.1094/Phyto-64-358.

Smiley, R. W., Gourlie, J. A., Easley, S. A. and Patterson, L. M., Pathogenicity of fungi associated with the wheat crown rot complex in Oregon and Washington. Plant Dis., 2005; 89(9): 949-957. https://doi.org/10.1094/PD-89-0949.

Covarelli, L., Beccari, G., Steed, A. and Nicholson, P., Colonization of soft wheat following infection of the stem base by Fusarium culmorum and translocation of deoxynivalenol to the head. Plant Pathol., 2012; 61(6), 1121-1129. https://doi.org/10.1111/j.1365-3059.2012.02600.x.

Revista Innovaciencia Facultad de Ciencias Exactas, Físicas y Naturales

Descargas

Publicado

2019-10-25

Cómo citar

Shareef Al-Tovi, B. S. ., & Abduljabbar Haleem, R. . (2019). Patogenicidad de especies de Fusarium asociados a daño del ápice y la raíz del trigo (Triticum aestivum) bajo diferentes condiciones. Innovaciencia, 7(1), 1–9. https://doi.org/10.15649/2346075X.659

Número

Sección

Artículo de investigación científica y tecnológica

Altmetrics

Descargas

Los datos de descargas todavía no están disponibles.