ew protocol of Tomato (Lycopersicon esculentum Mill.) in vitro propagation

Danial Gharbia H.; Ibrahim Diaa A.

doi:10.15649/2346075X.459

Authors

Danial Gharbia H. Gharbia H. Danial, College of Science/ Scientific Research Center /Duhok University
Ibrahim Diaa A. Diaa A. Ibrahim, College of Science/ Scientific Research Center /Duhok University

DOI:

https://doi.org/10.15649/2346075X.459

Keywords:

in vitro propagation, tomato, BAP, Zeatin.

Abstract

Introduction: A successful in vitro propagation system was developed for Sandra and Rocky cultivars of tomato plants (Lycopersicon esculentum Mill.) commonly grown in Kurdistan Region of Iraq by in vitro culture of shoot tips and node explants. Methods: Shoot tip and node explants were excised and cultured on basal MS medium containing several concentrations of BAP and Zeatin. Results: Multiple shoots formation of up to 2 shoots were obtained on MS medium supplemented with 2.0 mgl-1 Zeatin in node and shoot explants for the two cultivars. Microshoots were tested for root initiation on full MS medium supplemented with different concentrations of IBA, NAA and IAA. The best root formation was cultivar and 0.5 mgl-1 of NAA in rocky cultivar. Conclusion: The regenerated plants were successfully acclimatized and transplanted to the open field conditions.

Author Biographies

Danial Gharbia H., Gharbia H. Danial, College of Science/ Scientific Research Center /Duhok University

Gharbia H. Danial, College of Science/ Scientific Research Center /Duhok University

Ibrahim Diaa A. , Diaa A. Ibrahim, College of Science/ Scientific Research Center /Duhok University

Diaa A. Ibrahim, College of Science/ Scientific Research Center /Duhok University

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