Inhibición de genes de enterotoxinas de Staphylococcus aureususando extracto de plantas

Autores/as

  • Srwa A. Mohammed Assistant Prof. at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq
  • Mohammed I. J. Al-ani Assistant Lecturer at Koya University/Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq
  • Dereh L. Mohammed Plastic and reconstructive surgery Faruk Medical city Private Hospital Sulaimania, Iraq
  • Lina R. Salar Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq
  • Banaz M. Rasul Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

DOI:

https://doi.org/10.15649/2346075X.469

Palabras clave:

Staphylococcus aureus; Enterotoxin gene; Plasmid; Transformation.

Resumen

Introduction: Enterotoxigenic Staphylococcus aureus is an important pathogen that causes septicemia and bacteremia and is often associated with serious complications, such as endocarditis and osteomyelitis. Some Staphylococcus enterotoxins require only minute quantities to be toxic in humans. The present study focused on investigation how to remove this problematic issue. Objectives: This study was conducted to inhibit S. aureus enterotoxin genes that obtained from positive blood culture bottles of patients at the pediatric hospital in Sulaimania city. Methods: Twenty five isolates of S. aureus were isolated among 100 positive blood culture bottles and determined the strains that produce enterotoxins through culture method. Then, the enterotoxin genes that located on plasmids were cured by two medicinal plants (Eugenia caryophyllata and Cinnamomum zeylanicum). Results: The results showed that nine out of 25 isolates were released enterotoxins from which the plasmid encoding enterotoxin genes were confirmed in four of them. And, two of the isolates were transferred to recipient DH10B E. coli isolate successfully. Methanol extracts of (E. caryophyllata and C. zeylanicum) were used at sub minimum inhibition concentration as curing agents. Conclusion: Methanol extracts of (E. caryophyllata and C. zeylanicum) have grate effect on eliminating the plasmids
encoding enterotoxin gene of S. aureus.

Biografía del autor/a

Srwa A. Mohammed, Assistant Prof. at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Assistant Prof. at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Mohammed I. J. Al-ani, Assistant Lecturer at Koya University/Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Assistant Lecturer at Koya University/Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Dereh L. Mohammed, Plastic and reconstructive surgery Faruk Medical city Private Hospital Sulaimania, Iraq

Plastic and reconstructive surgery Faruk Medical city Private Hospital Sulaimania, Iraq

Lina R. Salar, Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Banaz M. Rasul, Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

Assistant Reasearcher at Koya University/ Faculty of Science and Health/ Medical Microbiology Department, Koya, Kurdistan region-Iraq

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Publicado

2018-12-28

Cómo citar

Mohammed, S. A. ., J. Al-ani, M. I., Mohammed, D. L., Salar, L. R. ., & Rasul, B. M. . (2018). Inhibición de genes de enterotoxinas de Staphylococcus aureususando extracto de plantas. Innovaciencia, 6(2), 1–8. https://doi.org/10.15649/2346075X.469

Número

Vol. 6 Núm. 2 (2018)

Sección

Artículo original de investigación e innovacion
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