Caracterización genotípica y nuevos tipos de secuencia multilocus de exoU+ de Pseudomonas aeruginosa presente en diferentes infecciones clínicas y entornos

Autores/as

  • Hemin E. Othman Dr.Hemin E. Othman, PhD of Molecular Microbiology,Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq
  • Eric L. Miller Dr. Eric L. Miller, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom
  • Jaladet MS. Jubrael ProfessorJaladet MS. Jubrael, Professor of Molecular biology, Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq
  • Ian S. Roberts Professor Ian S. Roberts,Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom

DOI:

https://doi.org/10.15649/2346075X.458

Palabras clave:

Pseudomonas aeruginosa; PCR; exoU; MLST; Phylogenetic diversity

Resumen

Introducción: The exoU gene, a marker for highly virulent strains of Pseudomonas aeruginosa, is the major contributor to a wide variety
of healthcare-associated infections. Methods: In this study, the antibiotic susceptibility profile, prevalence and genotyping of exoU+ P.
aeruginosa were demonstrated. A total of 101 isolates of P. aeruginosa were analysed from different clinical and environmental sources. Results: The antibiotic susceptibility profile classified these isolates as extensively drug resistant (35.6%), multidrug resistant (40.5%) and non-multidrug resistant (23.7%). The prevalence of exoU gene was screened by PCR and 23 exoU+ genotypes were detected which all were clinical isolates. Multilocus sequence typing (MLST) analysis of seven loci assigned these exoU+ genotypes to 21 sequence types (STs) from which 16 new STs were identified. The prevalent STs were ST-
308 and ST-235. Phylogenetic analysis using the concatenated nucleotide sequences of the seven housekeeping genes, exoU and the ITS region differentiated these exoU+ strains into five main groups. However, distinct evolutionary origins for some new sequence types were also indicated. Conclusions: The studied isolates showed the coexistence of exoU- and exoU+ genotypes of clinical P. aeruginosa in Kurdistan with a majority of MDR and XDR pattern. The prevalent STs found in other hospitals worldwide and at the international level.

Biografía del autor/a

Hemin E. Othman, Dr.Hemin E. Othman, PhD of Molecular Microbiology,Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq

Dr.Hemin E. Othman, PhD of Molecular Microbiology,Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq

Eric L. Miller, Dr. Eric L. Miller, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom

Dr. Eric L. Miller, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom

Jaladet MS. Jubrael, ProfessorJaladet MS. Jubrael, Professor of Molecular biology, Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq

ProfessorJaladet MS. Jubrael, Professor of Molecular biology, Scientific Research Centre, College of Science, University of Duhok, Kurdistan Region-Iraq

Ian S. Roberts, Professor Ian S. Roberts,Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom

Professor Ian S. Roberts,Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UnitedKingdom

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Revista Innovaciencia Facultad de Ciencias Exactas, Físicas y Naturales

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2018-12-28

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Othman, H. E. ., Miller, E. L. ., Jubrael, J. M., & Roberts, I. S. . (2018). Caracterización genotípica y nuevos tipos de secuencia multilocus de exoU+ de Pseudomonas aeruginosa presente en diferentes infecciones clínicas y entornos. Innovaciencia, 6(1), 1–14. https://doi.org/10.15649/2346075X.458

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