Detection of Alpha-1 antitrypsin and Interleukin-8 in meningitis patients
DOI:
https://doi.org/10.15649/2346075X.656Keywords:
Meningitis; IL-8; Alpha1antitrypsin; CSFAbstract
Meningitis is a meninges inflammation and the subarachnoid cerebrum spinal fluid that surrounds the spinal cord and brain. Infantile meningitis
is a major cause of morbidity & mortality in various parts of the world; it is earnest disorder owing to the nearness of the location to the brain and
spinal cord. It can be caused by virtually any organism; in general viral infections are more common than bacterial, fungal and parasitic infections
respectively. Objective: investigation of the Interleukin-8 (IL-8) and Alaph-1 Antitrypsin in the cerebrum spinal fluid of meningitis patients.
Material and Method: A study was done at the Medical city and Al-kadimia hospitals included 47 and 20 beings of Patients and Control groups
with an overall age range between 11 days to 6 years old. According to the causative agents of the disease, they were divided into three groups:
bacterial, viral and control groups. All subjects were monitored for the IL-8 by Enzyme Linked Immunosorbent Assay (ELISA) and Alaph-1
Anti-trypsin by Single Radial Immunodiffusion assay (SRIA) in the cerebrospinal fluid the data was analyzed via using SPSS (chi-squire test)
program (version 22). Results and discussion: The results revealed the presence of an elevation concentration of IL-8 among meningitis disease
patients (484.0695 pg/ml ), high concentration of IL-8 seemed in bacterial meningitis (825.3250pg/ml) when compared with its level in viral
meningitis (142.8143pg ml) ( p= 0.002) and control group ( 56.3409 pg/ml) (p=0.00) but no significant difference was found between viral and
control groups (p= 0.679). Alpha 1 antitrypsin results appeared high concentration of (21.6462 mg/ dl) and (16.4000 mg/ dl) in bacterial and viral
meningitis groups in sequentially, and that concentrations showed differences arrived significantly. The statistical analysis of this research showed
a correlation between IL-8 levels and both of neutrophil and lymphocyte in meningitis patients. IL-8 had significant correlation with lymphocyte
(p=0.02) in viral group while no significant correlation was appeared between IL-8 and neutrophil. In bacterial group, lymphocytes with IL-8 had
weak correlation, in contrary the correlation between neutrophil and IL-8 revealed the significantly correlation (p=0.01). No correlation was establish concerning the Alpha-1 antitrypsin and the cell’s numbers and types. Conclusion: A study indicated that Alpha 1 antitrypsin and IL-8 played
an important role in meningitis disease.
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