Effect of some physiological factors on citric acidproduction bythreeisolates ofAspergillus niger acid - hydrolyzed sawdust as a carbon source
DOI:
https://doi.org/10.15649/2346075X.477Keywords:
Sawdust hydrolysis, Citric acid, Aspergillus niger isolates.Abstract
Introduction: Citric acid (2-hydroxy-propane-1, 2, 3-tricarboxylic acid) was first isolated from lemon juice in 1784. It is a primary metabolic product which is formed in the tricarboxylic acid (Krebs) cycle. It is estimated that the market value of citric acid will exceed two billion dollars in 2019. About 70% of total citric acid produced globally is utilized in food industry, while about 12% is utilized in pharmaceuticals and cosmetic industries and the remainder in other industrial purposes. The industrial production of citric acid is undertaken by fermentation process in the presence of filamentous fungi for large scale of production. Aspergillus niger is the most efficient fungus due to its ability to produce more citric acid per unit time and ferment different inexpensive raw materials. Materials and Methods: Three isolates of the fungus Aspergillus niger (An1, An2, An3) were used throughout this study using different carbon source concentration in the form of sawdust acid hydrolysis supplemented with different concentration of (NH4)2H2SO4 as a nitrogen source. The effect of hydrogen ion concentration and addition of methanol to the fermentation medium was also investigated. Results and Discussion: The results indicated that the optimization of carbon and nitrogen concentration had stimulating
effect on citric acid production by the three used isolates. Moreover, addition of methanol at concentration of 1% at pH of 3.5 highly increased citric acid production. Conclusion: we concluded that the agriculture waste was a favorable substrate for the production of citric acid especially it is cost effective and easily obtainable.
References
Sauer, M., Porro, O., Matlanovich, D. and Branduardi, P. Microbial Production of Organic Acids. Expandingthe Markets.Trends in Biotechnology 2008; 26:100-108. https://doi.org/10.1016/j.tibtech.2007.11.006
Nadeem, A., Syed, Q., Baig, S.,Irfan, M. and Nadeem, M. Enhanced production of citric acid by Aspergillus niger M-101 using lower alcohols.Turkish Journal of Biochemistry, 2010; 35 (1):7-13.
Makut, M.D. & Adel-bijola, O.B. Citric acid producing fungi found in the soil environment of Keffi metropolis, Nasarawa state, Nigeria. International Research Journal of Microbiology, 2012; 3(7): 240-245.
Ali, S., Ul-haq, I., Qadeer, A. and Iqbal, J. Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor. Biotechnol. , 2002; 5 (Fulltext-3).https://doi.org/10.2225/vol5-issue3-fulltext-3
Aftab, N., Quratulain, S., Shahjahan, B. , Muhammad, I. and Muhammad, N. Enhancedproduction of citric acid by A niger M-101 using lower alcohols.Turk J. Biochem., 2010; 35(1): 7-13.
Thangavelu, R. and Murugaiyan, K. Citric acid production by using Gelidiella acerosa as a substrate. Indian J. Microbiol., 2011; 51(3): 289-293. https://doi.org/10.1007/s12088-011-0066-9
Prasad, M. P., Sridevi, V., Surendrababu, N.V. , Reddy, O.V. and Harsha,N. Studies on fermentative production of citric acid by Aspergillus niger isolate using sorghum malt and it's optimization. I. J. I. R. Sci. Eng.Technol., 2013; 2(7):2961-2968.
Igor. G. M., Svetlana, V. K. and Julia, N. L. Citric acid production by Yarrowia lipolytic yeast on different renewable raw materials. Fermentation, 2018; 4 (36): 2-7. https://doi.org/10.3390/fermentation4020036
Bachir, C. and Halima, C. Effect of adding sawdust on mechanical and physical properties of ceramic bricks to obtain light weight Building material. Inter. J. Mech. E., 2012; 6(11): 2521-2524.
Belen, M., Jose, M. S., Noelia, R. , Sandra, C. , Attilio, C. , Jose, M. D. (2010). Biotechnological production of citric acid. Brazil. J. Microl . , 2012; 41(4): 862-875. https://doi.org/10.1590/S1517-83822010000400005
Grewal, H. S. and Kalra, K. L. Fungal production of citric acid . Biotechnol.Adv., 1995; 13: 209-234. https://doi.org/10.1016/0734-9750(95)00002-8
Carlos, R. S. , Luciana, P. S. , Cristine, E. and Ashok, P. New perspectives for citric acid production and application. Food Technol. Biotechnol., 2006; 44(2): 141-149.
Roukas, T. and Kotzekidou, P. Pretreatment of data syrup to increase citric acidproduction. Enzyme and Microb. Technol. , 1997; 21: 273-276. https://doi.org/10.1016/S0141-0229(97)00041-0
Saha, M. L., Sakai, Y. and Takahashi, F. Ctric acid fermentation by magnetic drum contactor:use of methanol and ethnol for high production., J. Biosci. and Bioeng., 1999; 83(3): 394-396. https://doi.org/10.1016/S1389-1723(99)80053-X
Rugsaseel, S., Morikawa, S., Kirimura, k. and Usami, S. Stimulation of citric acidproduction in Aspergillus niger by addition of viscous substances in shake culture. APPL. Microb.Biotechnol., 1995; 42:839-843. https://doi.org/10.1007/s002530050340
Bhattacharjee, I. and Baruah, P. L. Isolation and screening of citric acid producing Aspergillus sp. and optimization of citric acid production by Aspergillus niger S-6 IORS . J. Enviro. Sci. Toxicology and food Technol., 2015; 9(3): 19-23.
Shankar, T. and Sivakumar, T. Screening of citric acid Producing fungi from the leaf litter soil of sathuragiri hills. Bioengineering and Bioscience, 2016; 4: 56 - 63.
Haider, M. M. Citric acid production from carob pod extract by Aspergillus niger . pharm. Bio. Sci., 2014; 9(3): 112-116. https://doi.org/10.9790/3008-0934112116
Pearson, D. Laboratory techniques in food analysis, London: Butterworth and Co. publishers Ltd. London, U.K, 1973.
Dubois, M. , Gilles, K. A. , Hamilton, J. K. , Rebers, P. A. and Ith, F. S. Calorimetricmethod for determination of sugars and related substances. Anal. Chem. , 1956; 28: 350 -356. https://doi.org/10.1021/ac60111a017
Sarangbin, S. and Watanapokasia, Y. Yam bean starch : A novel substrate for citric acid production by the protease , negative mutant strain of A niger. Carbohyd. Polym. , 1999; 38: 219-224. https://doi.org/10.1016/S0144-8617(98)00094-0
Jernejc, K. and Legisa, M. The influence of metal ions on malic enzyme activity and lipid synthesis in Aspergillus niger. FEMS Microbiol. Lett., 2002; 217: 185- 190. https://doi.org/10.1016/S0378-1097(02)01073-X
Kirimura, K.,Watanabe, T., Sunagawa, T. and Usami, S. Citric acid production from xylan and xylan hydrolysate by semi-solid culture. Biosic. Biotechnol. Biochem., 1995; 63(1): 226-228. https://doi.org/10.1271/bbb.63.226
Roukas, T. Citric acid production from carob pod extract by cell recycle of Aspergillus nigerATCC9142. Food Biotechnol., 1998; 12(1&2):91-104. https://doi.org/10.1080/08905439809549945
Nazz, A., Wadah, S., Sakhawat , A., Shahnaz, C. and Sana, l. Citric acid production from Aspergillus niger using Banana peel. I. J. Sci. E. R., 2015; 7(1): 868-872.
Haq, U. I., Sikander, A. and Iqbal, J. Stimulatory effect of alcohols (methanol and ethanol) on citric acid productivity by a 2-deoxy D-glucose resistant culture of Aspergillus niger GCB-47. Proc. Biochem., 2003; 39: 812-816. https://doi.org/10.1016/S0960-8524(02)00172-4
Maharani, V., Reeta, D. , Sundara, M. A., Vijaya lakshmi, S. and Balasubramania, K. Isolation and characterization of citric acid producing Aspergillus niger from spoiled coconut. I. J. Microbiol. Appl. Sci., 2014; 3(3):700-705.
Papagianni, M. , Mattey, M. and Kristiansen, B. Citric acid production and morphology of Aspergillus niger as functions of the maxing intensity in a stirred tank and a tuber loop bioreactor. Biochem. Eng. J., 1998; 2:197-205. https://doi.org/10.1016/S1369-703X(98)00032-1
Elgbory, S. Y. Acid hydrolysis of Kalghan plant (Silybum marianum) powder for the production of single cell protein by the yeast Candida utilis. M.Sc. Thesis, Mousl Unv. Mousl , Iraq, 1997.
Bozakouk, I. H. Acid hydrolysis of cane plant (Phragmites australis) powder for the production of single cell protein by the yeast Candida utilis . M.Sc. Thesis, Bengazi Univ. Bengazi, Libya, 2002.
Boominathan, S., Dhanaraj, T. S. and Muragaiah, K. Production and optimization of citric acid by Aspergillus niger using pineapple and tapioca waste. Herbal Tech. Industry, 2012; 12: 8-11.
Patil, N. G. and Patil, V. R. Production and partial characterization of citric acid by local isolate of Aspergillus niger using sorghum. I. j. Pharm., 2014; 5: 229-231.https://doi.org/10.7897/2230-8407.050349
Shetty, V. Production and optimization of citric acid by Aspergillus niger using molasses and corncob. I. J. pharm. and pharmaceutical Sci., 2015; 7(5): 152-157.
Prabha, S. M. and Rangaiah, S. G. Comparative study of citric acid production from Annona reticulate and it's peel with effect of alcoholas a stimulant. I. J. Biotechnol. 2014; 5(3):346-352.
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