MEJORAMIENTO ADAPTATIVO DE IMÁGENES PARA MICROSCOPIA DE FLUORESCENCIA
DOI:
https://doi.org/10.15649/2346075X.399Keywords:
Fluorescence, Bright field Microscopy, Digital images treatment.Abstract
Introduction: The light field microscope is a technique accessible to
majority of biological laboratories, as well as their adaptation to fluorescence. Due to a decrease in depth of field, several focalization planes are
present in which it can be confirmed that the sample has an appreciable volume. Materials and methods: Using the light field microscope,
with fluorescent substances that preserve the natural characteristics of the
specimen, make it possible to focus the sample in different parts of the
specimen at a measurable distance given by the fine focus (0.1 mm per rotation). Results and discussion: Points that are focused are related to the
displacement of the object (or the objective), acquiring focused and spatially localized planes, generating high contrast surfaces. Conclusions:
The dimensions of the sample are recognized; combining it with the optimization, obtains qualitative data of the same and the improvement in the
images obtained by the microscopy of clear field.
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